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Imielinski, M. et al. Mapping the hallmarks of lung adenocarcinoma with massively parallel sequencing. Cell 150, 1107–1120 (2012). Dorhoi, A. & Du Plessis, N. Monocytic myeloid-derived suppressor cells in chronic infections. Front. Immunol. 8, 1895 (2017). DeSantis, C. E., Kramer, J. L. & Jemal, A. The burden of rare cancers in the United States. Ca. Cancer J. Clin. 67, 261–272 (2017). Bolger, A. M., Lohse, M. & Usadel, B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114–2120 (2014).

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Angueira, A. R. et al. Defining the lineage of thermogenic perivascular adipose tissue. Nat. Metab. 3, 469–484 (2021). Li H, Liu D, Dai Y, Luan T, Yu S (2018) Personalized search over encrypted data with efficient and secure updates in mobile clouds. IEEE Trans Emerg Top Comput 6(1):97–109 Based on accumulated evidence in a century, GBC typifies chronic inflammation-associated cancers (CIACs) 4. The majority of GBCs reside in a harsh environment besieged by infectious (e.g., pathogens), chemical (e.g., pancreatic juice reflux), mechanical (e.g., gallstone irritation), metabolic (e.g., bile cholesterol supersaturation), and hydrodynamic (e.g., biliary sludge) stresses which, along with heightened cell division stress with aging, contribute to a multitude of microbial, cytotoxic, metabolic, genotoxic, and senescence-related inflammation processes 5, many of which have initiated and evolved from inflamed gallbladders for ~15 years or more 6.Ushijima, T., Clark, S. J. & Tan, P. Mapping genomic and epigenomic evolution in cancer ecosystems. Science 373, 1474–1479 (2021). Yamagiwa, H. & Tomiyama, H. Intestinal metaplasia-dysplasia-carcinoma sequence of the gallbladder. Acta Pathol. Jpn. 36, 989–997 (1986). Capkun S, Cagalj M, Srivastava MB (2006) Secure localization with hidden and mobile base stations. In: INFOCOM

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Chandran N, Goyal V, Moriarty R, Ostrovsky R (2014) Position-based cryptography. SIAM J Comput 43(4):1291–1341. https://doi.org/10.1137/100805005 Moekotte, A. L. et al. Gemcitabine-based adjuvant chemotherapy in subtypes of ampullary adenocarcinoma: international propensity score-matched cohort study. Br. J. Surg. 107, 1171–1182 (2020). RNA velocity was performed to investigate the potential inter-relationship of mesenchymal cell lineage using velocyto 89. BAM file containing all the mesenchymal cells was used in this pipeline, spliced/unspliced reads were annotated. The calculation of RNA velocity values for each gene in each cell and embedding RNA velocity vector to low-dimension space was done by following the velocyto pipeline. All the parameters were set as default. The result was visualized into UMAP plot. Tissue distribution preference of clusters Wang E, Rao T, Ben-zvi Ilan (2014) Enhancement of photoemission from and postprocessing ofK2CsSbphotocathode using excimer laser. Physical Review Special Topics - Accelerators and Beams. doi: 10.1103/physrevstab.17.023402

Li H, Yang Y, Dai Y, Bai J, Yu S, Xiang Y (2017) Achieving secure and efficient dynamic searchable symmetric encryption over medical cloud data. IEEE Trans Cloud Comput PP(99):1–1 Todoric, J. & Karin, M. The fire within: cell-autonomous mechanisms in inflammation-driven cancer. Cancer Cell 35, 714–720 (2019). El-Kebir, M., Satas, G. & Raphael, B. J. Inferring parsimonious migration histories for metastatic cancers. Nat. Genet. 50, 718–726 (2018). Changzhou Erdong Environmental Protection Technology Co., Ltd. is a professional manufacturer of biodegradable straws & biodegradable disposable tableware with 10 years of production experience To ensure each cell paired with a bead in a Gel Beads-in emulsion (GEM), 10× library preparation and sequencing beads with the unique molecular identifier (UMI) and cell barcodes were loaded close to saturation. Polyadenylated RNA molecules hybridized to the beads after exposure to the cell lysis buffer. Beads were retrieved into a single tube for reverse transcription. On cDNA synthesis, each cDNA molecule was tagged on the 5’ end with UMI and cell label indicating its cell of origin. Briefly, 10× beads were then subject to second-strand cDNA synthesis, adaptor ligation, and universal amplification. Sequencing libraries were prepared using randomly interrupted whole-transcriptome amplification products to enrich the 3’ end of the transcripts linked with the cell barcode and UMI. All the remaining procedures including the library construction were performed according to the standard manufacturer’s protocol (CG000206 RevD). Sequencing libraries were quantified using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 2100 and the Qubit High Sensitivity DNA Assay (ThermoFisher). The libraries were sequenced on NovaSeq6000 (Illumina) using 2 × 150 chemistry (Shanghai Bohao Biotechnology; Shanghai OEbiotech Corporation). DNA isolation and whole exon sequencing (WES)

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Swamy, M., Jamora, C., Havran, W. & Hayday, A. Epithelial decision makers: in search of the ‘epimmunome’. Nat. Immunol. 11, 656–665 (2010). Based on the idea that the hardness of covalent crystal is intrinsic and equivalent to the sum of the resistance to the indenter of each bond per unit area, a semiempirical method for the evaluation of hardness of multicomponent crystals is presented. Applied to beta-BC2N crystal, the predicted value of hardness is in good agreement with the experimental value. It is found that bond density or electronic density, bond length, and degree of covalent bonding are three determinative factors for the hardness of a polar covalent crystal. Our method offers the advantage of applicability to a broad class of materials and initializes a link between macroscopic property and electronic structure from first principles calculation. Unruh D (2014) Quantum position verification in the random oracle model. In: Advances in cryptology - CRYPTO 2014 - 34th annual cryptology conference. Proceedings, Part II, Santa Barbara, pp 1–18. https://doi.org/10.1007/978-3-662-44381-1-1 Genomic DNA of samples was extracted by QIAamp DNA Mini Kit (Qiagen) and then captured with an Agilent SureSelect Human All Exon Kit V6 (Agilent). DNA libraries were generated following the protocols provided by Illumina. DNA libraries were sequenced with the Illumina NovaSeq 6000 System (Illumina), yielding 150 bp of paired-end sequence, and FASTQ files were generated. The WES sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China). Single-cell RNA-seq data processing Key Laboratory of Metastable Materials Science and Technology, College of Materials Science and Engineering, Yanshan University, Qinhuangdao 066004, China.

Butler, A., Hoffman, P., Smibert, P., Papalexi, E. & Satija, R. Integrating single-cell transcriptomic data across different conditions, technologies, and species. Nat. Biotechnol. 36, 411–420 (2018). Bunin, A. et al. Protein tyrosine phosphatase PTPRS is an inhibitory receptor on human and murine plasmacytoid dendritic cells. Immunity 43, 277–288 (2015). Baryshev SV, Wang E, Jing C, Jabotinski V, Antipov S, Kanareykin AD, Belomestnykh S, Ben-Zvi I, Chen L, Wu Q, Li H, Sumant AV (2021) Cryogenic operation of planar ultrananocrystalline diamond field emission source in SRF injector. Applied Physics Letters 118:053505. doi: 10.1063/5.0013172

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Electron source, DC guns, Beam dynamics simulation, Energy recovery LINAC ,Beamline commissioning, Electron cooling ,and SRF gun Research Activities Biswas J, Wang E, Gaowei M, Liu W, Rahman O, Sadowski JT (2021) High quantum efficiency GaAs photocathodes activated with Cs, O2, and Te. AIP Advances 11:025321. doi: 10.1063/5.0026839 Flavahan, W. A., Gaskell, E. & Bernstein, B. E. Epigenetic plasticity and the hallmarks of cancer. Science 357 (2017).

Cheng, S. et al. A pan-cancer single-cell transcriptional atlas of tumor infiltrating myeloid cells. Cell 184, 792–809.e23 (2021). Electron-electron scattering (a), and electron-hole scattering (b). The solid/ring circles are the initial states of electrons/holes respectively, and the dotted rings are their end states after scattering. Reuse & Permissions Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014). The procedure of deparaffinization, rehydration, and endogenous peroxidases quench was conducted as standard IHC. In general, all incubation was conducted in a dark moisture chamber and at 300 rpm on an orbital shaker. Applied liquids were removed and washed three times with TBST after each step. Then one cycle of the multi-color staining protocol was performed according to the instructions (PerkinElmer). Images of immunofluorescence stained slides were acquired from the co-focal microscope using the 60× objective with saturation protection as a whole-slide overview. A spectral library was constructed with each OPAL-fluorochrome. The same spectral library was used for all analyzed immunofluorescence panels throughout the experiments. Tumor cell viability, apoptosis, and stemness assays Neyaz, A. et al. Clinical relevance of PD-L1 expression in gallbladder cancer: a potential target for therapy. Histopathology 73, 622–633 (2018).